Genetic modification of Brassica juncea
Khan Bangash, Sajid Ali / Khattak, Sahir Hameed / Swati, Zahoor Ahmad
Brassica juncea is one of the most important oilseed crop. We have developed an efficient and reproducible protocol for Agrobacterium-mediated transformation of B. juncea variety NIFA RAYA with Wasabi defensin gene to produce transgenic plants. This gene was isolated from the leaves of Wasabia japonica. We find out that co-cultivation of explants with the transformed agrobacterium inoculum having an OD ranged from 0.6-1 for 15-20 minutes give ...