Studies on lentiviral-mediated transgenesis
Sauvain, Marc-Olivier
Transgenic animals are essential research tools. Their generation through the injection of naked plasmid DNA into a fertilized oocyte has been the standard practice for almost 3 decades. However, this approach is invasive, largely limited to the mouse and results in low rates of transgene integration. Although the inefficiency of pronuclear injection can be overcome in small animals, this becomes economically more challenging in larger animals...