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This book provides a single-source reference on the current state of the ribosome profiling method by describing experimental protocols for the quantitative analysis of translation in a variety of model organisms. In addition, the volume presents a detailed overview of the existing software tools and includes detailed description of methods for statistical analysis, data processing, and visualization of ribosome profiling data. Written for the...

This book brings together a collection of methods for the successful detection, culture and investigation of H. pylori, and its associated diseases. Chapters guide the reader through overviews on H. pylori infection, protocols for analyzing human clinical samples, and protocols for characterizing H. pylori, its functional components and its interactions with host cells. Written in the highly successful Methods in Molecular Biology series forma...

This volume presents the latest methods and approaches used to better characterize the properties of the structural human brain, and also explores various dysfunctional and non-dysfunctional neuroanatomical variations. This book is divided into 3 parts: methods, non-clinical applications, and clinical applications. The chapters in Part 1 talk about methods to study cortical thickness and the general linear model. Part 2 covers changes in brain...

This volume explores the latest methods used to study AMPK by computational, biochemical, biophysical, cellular, and ex vivo and in vivo approaches. The chapters in this book cover specific topics, such as methods to measure change in cellular energy metabolism and analyze metabolic pathways regulated by AMPK, bioinformatics tools to identify AMPK targets, knockdown of AMPK by CRISPR-Cas9, production and crystallization of full-length human AM...

Understanding seed-related processes is of major social, environmental, and economic concern. The viability and vigor of seeds are the very basis for sustainable agriculture and forestry, and comprehending the molecular and cellular events underlying these processes will become increasingly important to many economical sectors and for species that provide the world’s food supply. Seed Dormancy: Methods and Protocols covers analytical methods a...