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Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology and includes methods that are among the most important and elucidating in the discipline, such as bioluminescent imaging of gene expressions, confocal im...

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers fluorescence fluctuation spectroscopy and includes chapters on such topics as Förster resonance energy transfer (fret) with fluctuation algorithms, protein corona on nanoparticles by FCS, and FFS approaches to the study of receptors in live cells.

These 42 entertaining, short reflections are not meant to offer the final word on the meaning of God, life, the universe and everything. Instead, they come from observation and ordinary, everyday experience. They offer the reader glimpses of a new perspective on daily life

Modern Minority presents a fresh examination of canonical and emergent Asian American literature's relationship to the genre of realism, particularly through its preoccupation with everyday life.

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Summerhill is a dog with a problem: he isn't exactly sure who he is. Living alone in a desolate world as its only inhabitant, he has no memories of his previous life-only the tantalizing clue that the answers he seeks may lie with a mysterious woman named Katherine, the hostess on a cruise ship that sails between dimensions.But Katherine has problems of her own, and if Summerhill wants her help in unlocking the secrets of his past, he'll have ...

In a world where aliens are the norm and humans are spreading out into the galaxy, Colly finds herself setting up an escort agency. The agency (for human females only) caters for sex with aliens and Colly is amongst the first to experience the many delights that await us. These tales are graphic in nature and are meant for adults only. All characters depicted are 18 or older. Enjoy

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Cake maker and single mom Claudia Bradley thinks she has life all figured out -- until her best friend Nate starts looking like a mouth-watering dessert and her son's father comes strolling back into the picture as if he'd never left ten years ago. Throw in a case of preteen angst and a family with lots of well-meant advice, and, well, she's whipping up a recipe for chaos! Nate West has always been there for Claudia, a basic ingredient in her ...

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Veteran acting coach Warren helps readers make the best of any script. She shows how to take big risks, deepen one's choices, perfect technique, and get hired.

Many books on screenwriting claim to have all the answers. Yet, quite often, the more aspiring writers read, the more confused they become by contradictory statements, incomplete perspectives, and methods that fail to do more than scratch the surface. Screenwriting Down to the Atoms was written to end the confusion. It picks up where the best leave off and makes up where the rest fall short by looking closer and digging deeper to reveal the tr...

In this groundbreaking work, the authors offer a refreshingly original guide to marriage that is grounded in experience, wisdom, and common sense.

Having lost half her body weight, Lizzie Hampton heads to her small hometown to test out her new body on an old flame. Just a harmless fling to get her self confidence back before she returns to the city and the new man in her life. But Lizzie's plan has a few bumps in the road.