Twenty eight actinomycetes strains were isolated from cotton plant rhizosphere soil of North Indian region of India. The isolates were identified as actinomycetes by morphological studies. Among them 11 strain are selected on basis of cellulolytic activities by primary screening. Cellulolytic activities were exhibited by strain A1, A2, A3, A8, A11, A13, A16, A18, Grey 72, AB1, AB4 and it was confirmed by formation of clear zone of hydrolysis around the colonies. Among the isolates maximum cellulolytic activity were exhibited by strains AB4 and it was determined by Enzyme assay.