Analytical ultracentrifugation consists of the application of a high gravitational field to a solution of particles and the real-time detection of the evolving spatial concentration gradients. When applied to macromolecules or nanoscopic particles, ultracentrifugation data can provide rich information on macromolecular shape, solvation, composition and size-distribution. This is the first comprehensive overview book on AUC to be published since the 1950s. This publication will enable newcomers to the field to master AUC without direct access to laboratories where it is practiced, paving the way for this powerful