This fully revised edition focuses on those processes that generate calcium signals, to offer the first comprehensive exploration of calcium signaling research from a methodological standpoint. Several new chapters have been added and in many cases, where chapters from the first edition were retained, new experts were recruited to offer a fresh perspective. Employing a comfortable format, this volume offers numerous research techniques, along with the pros and cons of each. This work contains invaluable information for established researchers, as well as for those graduate students and scientists just beginning to find a direction in cellular calcium signaling.