This book contains a compendium of 34 chapters of full information on current molecular biology and biotechnology principles, protocols, techniques and applications covering such topics such as , RNA isolation and RT-PCR procedure, Cloning of PCR DNA fragments: Preparation of fragments , dephosphorylation of vector and ligation steps, Transformation of competent cells, Minipreparation and restriction analysis of plasmids from transformed E. coli., Expression and purification of recombinant fusion protein, Detection of protein expression from cloned genes by western blotting, Purification of genomic DNA from corn shoots, Restriction enzyme digestion and gel electrophoresis of DNA and transfer of migrated DNA to a membrane by capillary action and many more.
This book will help you to have a current and up-to- date clear understanding of the principles, protocols and procedures used in the molecular biology laboratories in clinical molecular biology reference laboratories.