This third edition volume
expands on the previous editions both by presenting more detailed protocols for
the techniques described in the first and second editions of High Throughput
Screening: Methods and Protocols
and by covering important new procedures. The first chapter of
this book provides an overview of important assay development techniques, while
the rest of the chapters detail how to develop and execute screens at whatever
throughput the user needs. Some chapter examples are: structure-based virtual
screening,   high throughput screening using mass spectrometry, identification of state-dependent
blockers for voltage gated calcium channels, bioluminescence resonance
energy transfer platform to monitor protein-protein interactions in live cells,
high throughput flow cytometry, and application of imaging-based assays in microplate formats for high
content screening. Written in the highly successful Methods in Molecular
Biology series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and avoiding
known pitfalls.Cutting edge and thorough, High Throughput Screening: Methods and Protocols,
Third Edition, is a valuable resource for anyone who is interested in HTS
research.