Human Stem Cell Manual: A Laboratory Guide, Third Edition is a comprehensive compilation of ¿methods that work” for deriving, characterizing, and differentiating hPSCs, written by the researchers who developed and tested the methods and use them every day in their laboratories. The manual is much more than a collection of recipes, it is an extraordinary laboratory guide for both experienced stem cell research and those just beginning to use stem cells in their work. The editors chose a range of subjects that are critical to stem cell research worldwide and provide introductions for each chapter which discuss the importance of the information in the broad context of stem cell biology. Also included are chapters on the legal and ethical issues that affect stem cell research and researchers. Cross references in each chapter guide the reader to other chapters that may be of interest and emphasize the interrelatedness of the information in the book as a whole. New coverage includes disease modelling, drug development, and other cell therapy applications. The manual is written by foremost experts from around the world for anyone doing research in the field of stem cell biology.