This book constitutes the thorougly refereed proceedings of the Second International Conference on Information Technology in Bio- and Medical Informatics, ITBAM 2011, held in Toulouse, France, in August/September 2011, in conjunction with DEXA 2011.The 13 long papers and 5 short papers were carefully selected and address the following topics: decision support and data management in biomedicine, medical data mining and information retrieval, workflow management and decision support in medicine, and classification in bioinformatics. The papers show how broad the spectrum of topics in applications of information technology to biomedical engineering and medical informatics is.