The book deals with some of the important techniques of recombinant DNA technology including , isolation of plasmids and transformation of bacteria, isolation and characterization of nucleic acids, construction and screening of gene libraries, DNA sequencing, RFLP and RAPD gene mapping, genetic transformation and molecular analysis of the transgenic plants.The book includes protocols of some the important techniques currently in use in the field of plant biotechnology. The book also includes methods used in RFPL and RAPD analysis and a practical example of actual research problem in the field of plant genetic engineering. A glossary of important terms is also included which will be helpful to understand the subject matter. The preparation of common buffers and reagents used in the different protocols is included in the appendix.