Single cell analysis has typically been difficult and challenging to perform in such a way that meaningful statistical information can be extracted. This thesis documents the development of a microchip or lab-on-a-chip platform to perform single cell electrophoresis in parallel separation channels. It describes the use of optical tweezers to select a cell and transport it to a desired separation channel. It also describes the use of electromechanical shearing to lyse a cell. These two techniques are combined with laser-micromachined separation channels to perform simple electrohoresis on the fluorescently labeled cellular contents.
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ISBN | 9783838304489 |
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Sprache | eng |
Cover | Kartonierter Einband (Kt) |
Verlag | LAP Lambert Academic Publishing |
Jahr | 20100530 |
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