Wetland ecosystems are among the most productive ecosystems in the biosphere. Wetlands receive surface water inputs from streams (surface run off), precipitation and overland flow and subsurface water inputs from surface infiltration. Biodiversity refers to the variability among living organisms from all sources including inter alia, terrestrial, freshwater and marine aquatic ecosystems and the ecological complexes of which they are the part. Hosetti has described it as the library of life, i.e., variety of all genes, species of microorganisms, plants animals and ecosystems that are found on our planet. Phytoplankton has varied in physical and chemical requirements for population growth. Diatoms differ significantly with respect to motility, cell-wall composition and ornamentation, and nutritional and reproductive strategies. In present study we investigate relationship between Chlorophyll concentration and their impact on Microbial diversity with Physicochemical-Parameters. Sodav Bandharan is a temporary wetland near to Vellan Village, Kodinar.